Interactions between Salmonella typhimurium lipopolysaccharide and the antimicrobial peptide, magainin 2 amide

FEBS Letters

Blazyk, Jack¹  Rana, Fazale R.¹  Sultany, Catherine M.² 

[1] Department of Chemistry and College of Osteopathic Medicine Ohio University, Athens, OH 45701, USA;University Research and Instrumentation Center, Ohio University, Athens, OH 45701, USA

关键词: Magainin;    Lipopolysaccharide;    Phase change;    FT-IR spectroscopy;    NMR spectroscopy;    31P;    (Salmonella typhimurium);   

DOI  :  10.1016/0014-5793(90)80616-Q

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Effects of magainin 2 amide on the phase behavior of Salmonella typhimurium lipopolysaccharide were characterized by FT-IR spectroscopy. This antimicrobial cationic peptide disorders the lipopolysaccharide at molecular ratios of lipopolysaccharide to magainin greater than 4, and can induce a temperature-dependent structural reorientation. The nature of the five phosphate groups of lipopolysaccharide was determined by ³¹P NMR spectroscopy. At pH 7.4, the net charge on the phosphates is −7. Lipopolysaccharide undoubtedly plays an important role in modulating the interactions of magainin with the gram-negative cell envelope and may act as a molecular sponge to protect the plasma membrane.

【 授权许可】

Unknown