The rat β1 ‐subunit of the GABAA receptor forms a picrotoxin‐sensitive anion channel open in the absence of GABA

FEBS Letters

Malherbe, Pari²  Sigel, Erwin³  Möhler, Hanns¹  Baur, Roland³ 

[1] Institute of Pharmacology, University of Zürich, 8006 Zürich, Switzerland;Pharmaceutical Research Department, Hoffmann-La Roche, 4051 Basel, Switzerland;>Institute of Pharmacology, University of Bern, 3010 Bern, Switzerland

关键词: Aminobutyric acid;    γ-;    Aminobutyric acid receptor;    γ-;    Ion channel;    Picrotoxin;    Expression;    (Xenopus oocyte);   

DOI  :  10.1016/0014-5793(89)81576-5

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The structural basis of GABA-gated chloride channels in mammalian brain is presently explored by the functonal expression of cDNAs coding for the α, β or γ-subunits of the receptor and their isoforms. In this context, we expressed the cloned cDNA coding for the rat β₁-subunit of the GABA_A receptor in the Xenopus oocyte. Surprisingly, efficient expression of a functional ion channel was found. The channel was anion-selective, and able to open in the absence of GABA. Since this channel could be shut by the GABA-channel blocker picrotoxin, we conclude that the β₁ -subunit of the GABA_A receptor is sufficient to form binding sites for picrotoxin.

【 授权许可】

Unknown