Effects of vasoactive intestinal contractor (VIC) and endothelin on intracellular calcium level in neuroblastoma NG108‐15 cells

FEBS Letters

Mitsui, Youji³  Chang, Wei²  Ishida, Norio³  Fu, Tao¹  Saida, Kaname³  Okano, Yukio¹  Nozawa, Yoshinori¹ 

[1] Department of Biochemistry, Gifu University School of Medicine, Tsukasamachi-40, Gifu 500 Japan;Department of Neurosurgery, Gifu University School of Medicine, Tsukasamachi-40, Gifu 500 Japan;Cell Science and Technology Division, Fermentation Research Institute, Agency of Industrial Science and Technology, Tsukuba, Ibaraki 305, Japan

关键词: Vasoactive intestinal contractor;    Ca2+ level;    intracellular;    Endothelin-1;    Fura 2;    Inositol 1;    4;    5-triphosphate;    (Neuroblastoma NG108-15);    VIC;    vasoactive intestinal contractor;    ET;    endothelin-1;    VIP;    vasoactive intestinal polypeptide;    IP3(1;    4;    5);    inositol 1;    4;    5-trisphosphate;    PIP2;    phosphatidylinositol 4;    5-bisphosphate;    DMEM;    Dulbecco's modified Eagle's medium;   

DOI  :  10.1016/0014-5793(89)81569-8

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Effects on [Ca²⁺]_i levels of endothelin-1 (ET) and vasoactive intestinal contractor peptide (VIC), which is a novel member of the endothelin family, were examined in fura 2-loaded neuroblastoma NG108-15 cells. VIC was found to be a very effective stimulus for intracellular Ca²⁺ mobilization and to be more potent than ET. Intracellular calcium response to sequential addition of two stimulants exhibited the homologous desensitization of either ET or VIC, but no heterologous desensitization between ET and VIC. This indicates evidence suggesting that these two peptides act through distinct receptors.

【 授权许可】

Unknown