Development of a monoclonal antibody against recombinant neuroendocrine 7B2 protein

FEBS Letters

Martens, Gerard J.M.²  Ayoubi, Torik A.Y.²  van Duijnhoven, Hans L.P.¹  van de Ven, Wim J.M.¹  Timmera, Erika D.J.¹  Braks, Anneke A.M.²  Roebroek, Anton J.M.¹ 

[1] Dept of Biochemistry, University of Nijmegen, St. Adelbertusplein 1, 6525 EM Nijmegen, The Netherlands;Depts of Animal Physiology and Molecular Biology, University of Nijmegen, Toernooiveld, 6525 ED Nijmegen, The Netherlands

关键词: Protein;    7B2;    Monoclonal antibody;    Immunochemistry;    Protein;    neuroendocrine;    Protein;    prokaryotic hybrid;   

DOI  :  10.1016/0014-5793(89)81125-1

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Mouse monoclonal antibody MON-100 was raised against the neuroendocrine protein 7B2 using bacterially produced hybrid proteins. In Western blot analysis, MON-100 reacted with 3 different 7B2 hybrid proteins and not with the respective carrier proteins. Furthermore, MON-100 was reactive with recombinant 7B2 cleaved from a hybrid protein. In an immunohistochemical study, MON-100 exhibited strong reactivity with the intermediate lobe of the Xenopus pituitary gland, a tissue previously shown to contain 7B2 mRNA. Using MON-100, immunoprecipitation analysis of newly synthesized proteins produced by in vitro incubated Xenopus neurointermediate lobes revealed the biosynthesis of a single protein of M _r 24 kDa, the expected size of the 7B2 protein. It appears, therefore, that the anti-7B2 monoclonal antibody MON-100 can be successfully used for Western blot analysis and immunohistochemical analysis as well as for immunoprecipitation experiments.

【 授权许可】

Unknown