FEBS Letters | |
Development of a monoclonal antibody against recombinant neuroendocrine 7B2 protein | |
Martens, Gerard J.M.2  Ayoubi, Torik A.Y.2  van Duijnhoven, Hans L.P.1  van de Ven, Wim J.M.1  Timmera, Erika D.J.1  Braks, Anneke A.M.2  Roebroek, Anton J.M.1  | |
[1] Dept of Biochemistry, University of Nijmegen, St. Adelbertusplein 1, 6525 EM Nijmegen, The Netherlands;Depts of Animal Physiology and Molecular Biology, University of Nijmegen, Toernooiveld, 6525 ED Nijmegen, The Netherlands | |
关键词: Protein; 7B2; Monoclonal antibody; Immunochemistry; Protein; neuroendocrine; Protein; prokaryotic hybrid; | |
DOI : 10.1016/0014-5793(89)81125-1 | |
学科分类:生物化学/生物物理 | |
来源: John Wiley & Sons Ltd. | |
【 摘 要 】
Mouse monoclonal antibody MON-100 was raised against the neuroendocrine protein 7B2 using bacterially produced hybrid proteins. In Western blot analysis, MON-100 reacted with 3 different 7B2 hybrid proteins and not with the respective carrier proteins. Furthermore, MON-100 was reactive with recombinant 7B2 cleaved from a hybrid protein. In an immunohistochemical study, MON-100 exhibited strong reactivity with the intermediate lobe of the Xenopus pituitary gland, a tissue previously shown to contain 7B2 mRNA. Using MON-100, immunoprecipitation analysis of newly synthesized proteins produced by in vitro incubated Xenopus neurointermediate lobes revealed the biosynthesis of a single protein of M r 24 kDa, the expected size of the 7B2 protein. It appears, therefore, that the anti-7B2 monoclonal antibody MON-100 can be successfully used for Western blot analysis and immunohistochemical analysis as well as for immunoprecipitation experiments.
【 授权许可】
Unknown
【 预 览 】
Files | Size | Format | View |
---|---|---|---|
RO201912020292566ZK.pdf | 741KB | download |