Epitope mapping of the monoclonal antibody FAC2 on the apoprotein of CPa‐1 in photosystem II

FEBS Letters

Bricker, Terry M.¹  Frankel, Laurie K.¹ 

[1] Department of Botany, Louisiana State University, Baton Rouge, LA 70803, USA

关键词: Photosystem II;    Protein;    CPa-1;    Monoclonal antibody;    Epitope mapping;   

DOI  :  10.1016/0014-5793(89)81552-2

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Using a combination of cyanogen bromide cleavage and endoproteinase digestion we have shown that the putative epitope for the monoclonal antibody FAC2 lies in the region ³⁶⁰Pro-₃₉₁Ser on the apoprotein of CPa-1. This region lies entirely within the large extrinsic loop of this protein. We have shown previously that the epitope of FAC2 becomes exposed in oxygen-evolving membranes upon treatment with alkaline Tris which releases all four of the manganese associated with the oxygen-evolving site of photosystem II. The epitope is not exposed, however, after CaCl₂ treatment and exposure to low concentrations of chloride, conditions which lead to the release of two of the four manganeses associated with the oxygen-evolving site [(1987) Arch. Biochem. Biophys. 256, 295–301]. These results suggest that, upon release of the chloride-insensitive manganese from photosystem II membranes, a conformational change occurs which leads to the exposure of ³⁶⁰Pro-³⁹¹Ser on CPa-1 to the monoclonal antibody FAC2.

【 授权许可】

Unknown