Alzheimer's disease: Microtubule‐associated proteins 2 (MAP 2) are not components of paired helical filaments

FEBS Letters

Mazie, J.C.²  Rosemblatt, M.³  Defossez, A.¹  Fellous, A.⁴  Delacourte, A.¹ 

[1] Institut Pasteur (Hybridolab) and INSERM U16 and U 156, ADERMA, Faculté de Médecine, 59045 Lille, France;Unidad de Immunologia Celular, INTA Universidad de Chile, Casilla 15138, Santiago, Chile;INSERM U 96, Unité de Recherche sur la Grande Thyroide et la Regulation Hormonal, Le Kremlin-Bicêtre 94275 Cedex, France

关键词: Alzheimer's disease;    Neurofibrillary tangle;    Microtubule-associated protein 2;    Paired helical filament;    NFT;    neurofibrillary tangles;    PHF;    paired helical filaments;    NP;    neuritic plaque;    mAb;    monoclonal antibody;    PMSF;    phenylmethylsulfonyl fluoride;    MAPs;    microtubule-associated proteins;   

DOI  :  10.1016/0014-5793(89)80895-6

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

In Alzheimer's disease, the most characteristic neuropathological changes are the formation of neurofibrillary tangles (NFT) and neuritic plaques (NP) characterized by the presence of bundles of paired helical filaments (PHF) that accumulate in the degenerating neurites and neuronal cell bodies. Although the protein composition of the PHF is ill-defined, a number of microtubule-associated proteins have been implicated in these lesions. Here we report results with an antiserum monospecific for the microtubule-associated protein MAP 2 which does not cross-react with any other microtubular protein. Immunostaining with this antibody of sections from an Alzheimer's brain show a strong reactivity with NFT but no reactivity at the level of the NP. On the other hand, immunostaining of Alzheimer's brain sections with another antibody specific for the microtubule-associated protein τ shows strong staining of PHF on both NFT and NP. These findings confirm the presence of the τ proteins in the PHF and strongly suggest that MAP 2 may not be a main structural component of the PHF. Labelling of NFT with the anti-MAP 2 antiserum suggests a non-specific binding of MAP 2 to the PHF during the process of NFT formation.

【 授权许可】

Unknown