Tentative identification of RNA‐dependent RNA polymerases of dsRNA viruses and their relationship to positive strand RNA viral polymerases

FEBS Letters

Chumakov, K.M.¹  Koonin, E.V.¹  Gorbalenya, A.E.² 

[1] Institute of Microbiology, USSR Academy of Sciences, Moscow, USSR;Institute of Poliomyelitis and Viral Encephalitides, USSR Academy of Medical Sciences, Moscow Region, USSR

关键词: Sequence comparison;    dsRNA-containing virus;    RNA virus;    positive-strand;    RNA-dependent RNA polymerase;    Virus evolution;    CV;    coxsackie virus type B4;    EMCV;    encephalomyocarditis virus;    FMDV;    foot-and-mouth disease virus;    TEV;    tobacco etch virus;    SNBV;    Sindbis virus;    WNV;    West Nile virus;    TRV;    tobacco rattle virus;    CMV;    cucumber mosaic virus;    CarMV;    carnation mottle virus;    BNYVV;    beet necrotic yellow vein virus;    IBV;    infectious bronchitis virus;    GA;    GA bacteriophage (positive strand RNA viruses);    IBDV;    infectious bursa disease virus;    φ6;    φ6 bacteriophage;    REO;    reovirus;    BTV;    bluetongue virus;    ScV;    Saccharomyces cerevisiae virus (dsRNA viruses);   

DOI  :  10.1016/0014-5793(89)80886-5

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Amino acid sequence stretches similar to the four most conserved segments of positive strand RNA viral RNA-dependent RNA polymerases have been identified in proteins of four dsRNA viruses belonging to three families, i.e. P2 protein of bacteriophage φ6 (Cystoviridae), RNA 2 product of infectious bursa disease virus (Birnaviridae), λ3 protein of reovirus, and VP1 of bluetongue virus (Reoviridae). High statistical significance of the observed similarity was demonstrated, allowing identification of these proteins as likely candidates for RNA-dependent RNA polymerases. Based on these observations, and on the previously reported sequence similarity between the RNA polymerases of a yeast dsRNA virus and those of positive strand RNA viruses, a possible evolutionary relationship between the two virus classes is discussed.

【 授权许可】

Unknown