Synergistic enhancement of type I and III collagen production in cultured fibroblasts by transforming growth factor‐β and ascorbate

FEBS Letters

Johnston, Dennis A.¹  O'Brien, Wanda R.³  Appling, W.Douglas²  Duvic, Madeleine³ 

[1] Department of Biomathematics, University of Texas M. D. Anderson Cancer Center, Houston, TX 77030, USA;Division of Facial Plastic and Reconstructive Surgery, Department of Otorhinolaryngology and Communicative Sciences, Baylor College of Medicine, Houston, TX 77030, USA;Departments of Dermatology and Internal Medicine, University of Texas Medical School and University of Texas M. D. Anderson Cancer Center, Houston, TX 77030, USA

关键词: Transforming growth factor-β;    Ascorbate;    Vitamin C;    Collagen;    (Dermal fibroblast);   

DOI  :  10.1016/0014-5793(89)80792-6

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Transforming growth factor-β (TGF-β) is a prototype of a family of polypeptides that regulates cellular growth and phenotypic differentiation [(1986) Science 233, 532-534; (1987) Cell 49, 437-438]. TGF-β injection induces angiogenesis and fibrosis locally [(1986) Proc. Natl. Acad. Sci. USA 83, 4167-4171; (1987) Science 237, 1333-1336] and stimulates the synthesis of extracellular matrix proteins, fibronectin, collagens, and proteoglycans in vitro in many cell types [(1986) J. Biol. Chem. 261, 4337-4345; (1987) Biochem J. 247, 597-604]. Ascorbate is also known to induce collagen synthesis and to promote wound healing [(1988) J. Invest. Dermatol. 90, 420-424; (1986) Coll. Rel. Res. 6, 455-466]. We report that in cultured human skin fibroblasts, ascorbate and TGF-β synergistically enhance the biosynthesis of type I and III collagens and their steady-state mRNAs. TGF-β alone has no enhancing effect on type III collagen synthesis. The cooperation between ascorbate and TGF-β may be of significance in wound healing and in disorders of fibrosis.

【 授权许可】

Unknown