CD3/T‐cell receptor coupling to a pertussis and cholera toxin‐insensitive G‐protein

FEBS Letters

Jondal, Mikael¹  Fredholm, Bertil B.²  Kvanta, Anders²  Nordstedt, Christer²  van der Ploeg, Ingeborg² 

[1] Dept of Immunology, Box 60400, Karolinska Institutet, 104 01 Stockholm, Sweden;Dept of Pharmacology Box 60400, Karolinska Institutet, 104 01 Stockholm, Sweden

关键词: T-lymphocyte;    Signal transduction;    GTP hydrolysis;    GTP binding;    PLC;    phospholipase C;    cAMP;    cyclic adenosine 3′;    5′-monophosphate;   

DOI  :  10.1016/0014-5793(89)80791-4

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

We have analyzed the effect of CD3/T-cell receptor stimulation on GTP hydrolysis and GTP binding. We show that stimulation of Jurkat, T-cell, membranes withT3 results in a 50% increase in GTP hydrolysis which is specifically inhibited by GDP. Pretreatment of the membranes with neither pertussis toxin nor cholera toxin inhibited the GTP hydrolysis. We also show that stimulation withT3 increases the binding of GTPγS to Jurkat membranes. These data strongly implicate the involvement of a G-protein in CD3/T-cell receptor signalling.

【 授权许可】

Unknown