Direct incorporation of microtubule oligomers at high GTP concentrations

FEBS Letters

Burns, Roy G.¹  Islam, Khalid¹ 

[1] Biophysics Section, Department of Physics, Imperial College of Science and Technology, London SW7 2BZ, England

关键词: Microtubule assay;    GTP binding;    Tubulin oligomer;   

DOI  :  10.1016/0014-5793(84)81019-4

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Chick brain microtubule protein consists primarily of a mixture of MAP2 : tubulin oligomers and dimeric tubulin. The assembly of this protein is described by a single pseudofirst-order reaction at 20 μM GTP, but by the summation of two pseudofirst-order reactions at 1 mM GTP. The protein contains two GTP-binding species, corresponding to the tubulin dimers and the oligomers, and conditions which alter the dimer : oligomer equilibrium, affect the kinetics of microtubule assembly. The results indicate that the oligomers are only direct assembly intermediates at high GTP concentrations.

【 授权许可】

Unknown