GTP interacts with the γ‐subunit of eukaryotic initiation factor eIF‐2

FEBS Letters

Karpova, G.G.¹  Babkina, G.T.¹  Bommer, U.-A.¹  Kurzchalia, T.V.¹ 

[1] Central Institute of Molecular Biology, Academy of Sciences of the GDR, GDR-1115 Berlin-BuchUSSR

关键词: Photoaffinity labeling;    GTP analog;    GTP binding;    Initiation factor-2;    (Rat liver);   

DOI  :  10.1016/0014-5793(84)80758-9

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Eukaryotic initiation factor eIF-2 is an oligomeric protein consisting of three different subunits. During initiation of protein synthesis eIF-2 interacts with GTP, Met-tRNA_f and 40 S ribosomal subunit. By affinity labeling with a photo-reactive GTP analogue it was shown that in the binary complex [eIF-2 · GTP] GTP is in contact with the γ-subunit of eIF-2.

【 授权许可】

Unknown