Phorbol ester treatment impairs hormone‐ but not stable GTP analog‐induced inhibition of adenylate cyclase

FEBS Letters

Jakobs, Karl H.¹  Bauer, Silvia¹ 

[1] Pharmakologisches Institut der Universität Heidelberg, Im Neuenheimer Feld 366, D-6900 Heidelberg, FRG

关键词: Adenylate cyclase;    Inhibitory coupling protein Ni;    Phorbol ester;    Protein kinase C Inhibitory hormone;    GTP analog;   

DOI  :  10.1016/0014-5793(86)81181-4

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Treatment of intact human platelets and S49 lymphoma cyc⁻ cells with the phorbol ester, 12-O-tetradecanoylphorbol-13-acetate, impairs GTP-dependent and hormone-induced inhibition of adenylate cyclase, an action mediated by the inhibitory coupling protein N_i. In contrast, receptor-independent activation of N_i with subsequent adenylate cyclase inhibition induced by the stable GTP analog, guanosine 5'-[γ-thio]triphosphate, was affected in neither the potency nor onset of N_i activation by the stable GTP analog, in both membrane systems studied. The data indicate that modification of N_i following phorbol ester treatment does not impair its activation by stable GTP analogs.

【 授权许可】

Unknown