Phorbol ester inhibits myoblast fusion and activates β‐adrenergic receptor coupled adenylate cyclase

FEBS Letters

Johnson, Dennis D.²  Wilcox, Robert²  Phan, Ngoc T.¹  Sulakhe, Prakash V.¹ 

[1] Departments of Physiology, College of Medicine, University of Saskatchewan, Saskatoon, Saskatchewan S7N OWO, Canada;Departments of Pharmacology, College of Medicine, University of Saskatchewan, Saskatoon, Saskatchewan S7N OWO, Canada

关键词: Myoblast;    Adenylate cyclase;    Phorbol ester;    Fusion;    Creatine kinase;    β-Adrenergic receptor;   

DOI  :  10.1016/0014-5793(85)80725-0

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Primary cultures of myoblasts, derived from embryonic chick pectoral muscle, were treated with phorbol ester (TPA) for 8–96 h. TPA treatment blocked the fusion of myoblasts along with the expression of the MM form of creatine kinase. Interestingly, TPA treatment markedly increased the activity of β-adrenergic receptor coupled adenylate cyclase (AC) activity. The study suggests that TPA treatment augments the functional interaction between a coupling N_s protein and catalytic unit of AC. The likely significance of these results is briefly presented.

【 授权许可】

Unknown