FEBS Letters | |
Characterization of DNA rearrangements of N‐myc gene amplification in three neuroblastoma cell lines by pulsed‐field gel electrophoresis | |
Hashimoto, Keiichiro1  Kurosawa, Yoshikazu1  Kishikawa, Teruaki2  Okamura, Kazuhiko1  Kato, Hiroshi1  | |
[1] Institute for Comprehensive Medical Science Fujita-Gakuen Health University, Toyoake, Aichi 470-11, Japan;Department of Surgery, Fujita-Gakuen Health University, Toyoake, Aichi 470-11, Japan | |
关键词: Alu sequence; Gene amplification; Neuroblastoma; Gene; N-myc; Pulsed-field gel electrophoresis; DHFR; dihydrofolate reductase; HSR; homogeneously staining region; PFG; pulsed-field gel electrophoresis; | |
DOI : 10.1016/0014-5793(89)80790-2 | |
学科分类:生物化学/生物物理 | |
来源: John Wiley & Sons Ltd. | |
【 摘 要 】
We characterized N-myc gene amplification in three human neuroblastoma cell lines (IMR-32, TGW, GOTO). Rearrangements in long-range regions surrounding amplified N-myc genes were examined by pulsed-field gel electrophoresis. Since rare-cutting enzymes completely digested DNA at the middle of the N-myc gene, we were able to construct a physical map upstream and downstream of the germline N-myc gene, and to obtain information on restriction sites surrounding amplified N-myc genes. This method enables us to envisage the organization of amplified units over a long range. Digestion patterns differed considerably among the germline and the three cell lines, but were simple in each case. We estimated that the minimal distance between neighboring N-myc genes is at least several hundred kilobases. Our data suggest that amplification units contain several DNA fragments derived from ditterent loci, but that they are homogeneous.
【 授权许可】
Unknown
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