Characterization of DNA rearrangements of N‐myc gene amplification in three neuroblastoma cell lines by pulsed‐field gel electrophoresis

FEBS Letters

Hashimoto, Keiichiro¹  Kurosawa, Yoshikazu¹  Kishikawa, Teruaki²  Okamura, Kazuhiko¹  Kato, Hiroshi¹ 

[1] Institute for Comprehensive Medical Science Fujita-Gakuen Health University, Toyoake, Aichi 470-11, Japan;Department of Surgery, Fujita-Gakuen Health University, Toyoake, Aichi 470-11, Japan

关键词: Alu sequence;    Gene amplification;    Neuroblastoma;    Gene;    N-myc;    Pulsed-field gel electrophoresis;    DHFR;    dihydrofolate reductase;    HSR;    homogeneously staining region;    PFG;    pulsed-field gel electrophoresis;   

DOI  :  10.1016/0014-5793(89)80790-2

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

We characterized N-myc gene amplification in three human neuroblastoma cell lines (IMR-32, TGW, GOTO). Rearrangements in long-range regions surrounding amplified N-myc genes were examined by pulsed-field gel electrophoresis. Since rare-cutting enzymes completely digested DNA at the middle of the N-myc gene, we were able to construct a physical map upstream and downstream of the germline N-myc gene, and to obtain information on restriction sites surrounding amplified N-myc genes. This method enables us to envisage the organization of amplified units over a long range. Digestion patterns differed considerably among the germline and the three cell lines, but were simple in each case. We estimated that the minimal distance between neighboring N-myc genes is at least several hundred kilobases. Our data suggest that amplification units contain several DNA fragments derived from ditterent loci, but that they are homogeneous.

【 授权许可】

Unknown