Cloning of Alu‐containing cDNAs from human fibroblasts and identification of small Alu+ poly(A)+ RNAs in a variety of human normal and tumor cells

FEBS Letters

Maleeva, Natalia E.²  Ryskov, Alexei P.¹  Jincharadze, Appolon G.¹  Korneev, Sergei A.²  Limborska, Svetlana A.²  Ivanov, Pavel L.¹  Slominsky, Petr A.² 

[1] Institute of Molecular Biology, USSR Academy of Sciences, Vavilov str. 32, 117984, Moscow B-334, USSR;Institute of Molecular Genetics, USSR Academy of Sciences, Kurchatov Sq. 46, Moscow 123182 USSR

关键词: Alu sequence;    cDNA;    Cloning;    Transcription;    (Human);   

DOI  :  10.1016/0014-5793(87)81346-7

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Two clones have been selected from a human fibroblast cDNA bank. By DNA sequencing the clones were shown to contain Alu elements located near the ends of the cDNA inserts. DNA of the clones was used for Northern blot hybridization analysis of a number of poly(A)-containing RNAs from normal human tissues (brain, stomach, uterus, spleen, fibroblasts) and tumors (neurinoma, glioma, neuroblastoma, liposarcoma, adrenal cortex adenocarcinoma). All RNA samples reveal a heterodisperse distribution of Alu transcripts with discrete bands in the region of 7-12 S RNA. The majority of these small poly(A)⁺ Alu⁺ RNAs contain Alu sequences only in one (canonical) orientation with functional signals including the split promoter for RNA polymerase III.

【 授权许可】

Unknown