Regulation of colominic acid biosynthesis by temperature: Role of cytidine 5′‐monophosphate N‐acetylneuraminic acid synthetase

FEBS Letters

Reglero, Angel¹  Luengo, José M.¹  González-Clemente, Camino¹  Rodríguez-Aparicio, Leandro B.¹ 

[1] Department of Biochemistry and Molecular Biology, University of León, ‘Campus de Vegazana’, 24071 León, Spain

关键词: Colominic acid;    CMP-N-acetylneuraminic acid synthetase;    (E. coli);   

DOI  :  10.1016/0014-5793(89)80770-7

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Synthesis of colominic acid in Escherichia coli K-235 is strictly regulated by temperature. Evidence for the role of cytidine 5′-monophospho-N-acetylneuraminic acid (CMP-Neu5Ac) synthetase in this regulation was obtained by measuring its level in E. coli grown at 20 and 37°C. No activity was found in E. coli grown at 20°C. CMP-Neu5Ac started to be quickly synthesized when bacteria grown at 20°C were transferred to 37°C and was halted when cells grown at 37°C were transferred to 20°C. These findings suggest that temperature regulates the synthesis of this enzyme and therefore the concentration of CMP-Neu5Ac necessary for the biosynthesis of colominic acid.

【 授权许可】

Unknown