SecA protein is directly involved in protein secretion in Escherichia coli

FEBS Letters

Matsuyama, Shin-ichi¹  Sasaki, Shoichi¹  Mizushima, Shoji¹  Akita, Mitsuru¹  Kawasaki, Hisashi¹ 

[1] Institute of Applied Microbiology, The University of Tokyo, Yayoi, Bunkyo-ku, Tokyo 113, Japan

关键词: Protein;    SecA;    Protein overproduction;    Protein secretion;    (E. coli);   

DOI  :  10.1016/0014-5793(89)80516-2

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

A high-expression plasmid for the secA gene was constructed. The SecA protein was then overproduced in E. coli and purified. The purified SecA stimulated the in vitro translocation of a model secretory protein into inverted membrane vesicles pretreated with 4 M urea. Membrane vesicles from a secA^ts mutant exhibited lower translocation activity, which was enhanced by SecA. These results indicate that SecA is directly involved in protein secretion across the cytoplasmic membrane.

【 授权许可】

Unknown