Modulation by somatostatin of nerve‐mediated activation of glycogenolysis in the perfused rat liver

FEBS Letters

Arnold, Rudolf¹  Beckh, Karlheinz¹  Ehlenz, Klaus¹ 

[1]Department of Internal Medicine, Philipps-University, Marburg, FRG

关键词: Perfused liver;    Noradrenaline;    Somatostatin;    Carbohydrate metabolism;    (Rat;    Hepatic nerve);    HPLC;    high-pressure liquid chromatography;    VIP;    vasoactive intestinal polypeptide;    Glucose dehydrogenase (EC 1.1.1.47);    glutamate-pyruvic transaminase (EC 2.6.1.2);    lactate dehydrogenase (EC 1.1.1.27);   

DOI  :  10.1016/0014-5793(89)80752-5

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Perivascular nerve stimulation of rat livers perfused in situ with erythrocyte-free Krebs-Henseleit buffer at constant pressure in a non-recirculating system resulted in an increase of glucose and lactate production and in a decrease of portal flow. Infusion of somatostatin in different concentrations (2 × 10⁻⁷, 10⁻⁸, 10⁻⁹ mol·l⁻¹) reduced the nerve-mediated activation of glucose release maximally to 66%. There was only a slight effect on the lactate output, the nerve-mediated reduction of portal flow was unaltered. In controls, somatostatin alone had no effect on the metabolic and hemodynamic parameters. In order to differentiate between a presynaptic and postsynaptic mechanism, the noradrenaline overflow was calculated. The unaltered release of the neurotransmitter in the presence or absence of somatostatin excluded a presynaptic mechanism. To mimic the nerve effects on the carbohydrate metabolism and on the hemodynamics, noradrenaline (2 × 10⁻⁷ mol·l⁻¹) was infused instead of the nerve stimulation over a period of 5 min. Somatostatin did not change the endocrine effects of the catecholamine under these conditions. The nerve-dependent effect of somatostatin suggests that other neurotransmitters (e.g. VIP) or mediators (e.g. prostanoids) may be influenced by somatostatin.

【 授权许可】

Unknown