An in vitro demonstration of peroxisome proliferation and increase in peroxisomal β‐oxidation system mRNAs in cultured rat hepatocytes treated with ciprofibrate

FEBS Letters

Usman, Mohammed I.¹  Mangino, Mario¹  Rao, M.Sambasiva¹  Reddy, Janardan K.¹  Alvares, Keith¹  Thangada, Shobha¹ 

[1]Department of Pathology, Northwestern University Medical School, Chicago, IL 60611, USA

关键词: Peroxisome proliferation;    mRNA;    Tissue culture;    (Rat hepatocyte);   

DOI  :  10.1016/0014-5793(89)80721-5

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Using the normal adult rat hepatocytes, plated on rat tail collagen-coated dishes and fed a chemically defined medium, we demonstrate here that ciprofibrate at 0.1 mM concentration, increases significantly the mRNA levels of fatty acyl-CoA oxidase, enoyl-CoA hydratase/3-hydroxyacyl-CoA dehydrogenase bifunctional protein, and thiolase (the three enzymes of the β-oxidation system), and causes peroxisome proliferation. Increase in mRNA levels of these genes was evident within 1 h and was maximal 24 h after the addition of ciprofibrate. In hepatocytes cultured in the absence of ciprofibrate, the basal levels of these enzymes were low and further declined with time. Concomitant treatment of hepatocytes with cycloheximide did not inhibit or superinduce the mRNA levels, indicating that this induction may represent a primary (direct) effect of this compound on the expression of these genes and does not apparently involve short-lived repressor protein(s).

【 授权许可】

Unknown