Is the cytosolic catalase induced by peroxisome proliferators in mouse liver on its way to the peroxisomes?

FEBS Letters

Lundgren, Bo¹  Andersson, Karin¹  DePierre, Joseph W.¹  Eriksson, Anna Messing¹ 

[1]Unit for Biochemical Toxicology, Department of Biochemistry, Wallenberg Laboratory, Stockholm University, S-106 91 Stockholm, Sweden

关键词: Catalase;    Peroxisome proliferation;    Clofibrate;    Nafenopin;    WY-14.643;    Liver;    Mouse;   

DOI  :  10.1016/0014-5793(92)81276-R

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Dietary treatment of male C57B1/6 mice with clolibrate, nafenopin or WY-14.643 resulted in a modest (at most 2-fold) increase in the total catalase activity in the whole homogenate and mitochondrial fraction prepared from the livers of these animals. On the other hand, the catalase activity recovered in the cytosolic fraction was increased 12- to 18-fold, i.e. 30–35% of the total catalase activity in the hepatic homogenate was present in the high-speed supernatant fraction after treatment with these peroxisome proliferators. A study of the time course of the changes in peroxisomal and cytosolic catalase activities demonstrated that the peroxisomal activity both increased upon initiation of exposure and decreased after termination of treatment several days after the increase and decrease, respectively, in the corresponding cytosolic activity. This finding suggests that the cytosolic catalase may be on its way to incorporation into peroxisomes.

【 授权许可】

Unknown