Cloning and sequencing of the human nucleolin cDNA

FEBS Letters

Pollard, Harvey B.¹  Burns, A.Lee¹  Srivastava, Meera²  Fleming, Patrick J.² 

[1] Laboratory of Cell Biology and Genetics, National Institute of Diabetes, Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD 20892, USA;Department of Biochemistry, Georgetown University School of Medicine, Washington, DC 20007, USA

关键词: Nucleolin;    Sequence;    Retina;    Homology;   

DOI  :  10.1016/0014-5793(89)80692-1

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

A cDNA containing the entire coding region for human nucleolin has been isolated from a λ gt10 human retinal library using a bovine cDNA probe. The cDNA hybridized to a transcript of 3000 bases from fast-dividing cells, as well as terminally differentiated tissues of several species. Translation of the nucleotide sequence revealed a long open reading frame which predicts a 707 amino acid protein with several distinct domains. These include repeating elements, four conserved RNA-binding regions, a glycine-rich carboxy-terminal domain and sites for phosphorylation, glycosylation and dibasic cleavage. Human and bovine nucleolin exhibited more additions and/or substitutions of aspartate, glutamate and serine residues in the chromatin-binding domains by comparison with the hamster and mouse nucleolins. These differences may be related to species-specific functions in transcription.

【 授权许可】

Unknown