Chemical characterization of cyanogen bromide fragments from the β‐chain of human complement factor C3

FEBS Letters

Lundwall, Åke¹  Hellman, Ulf¹  Eggertsen, Gösta¹  Sjöquist, John¹ 

[1] Department of Medical and Physiological Chemistry, Box 575, Uppsala University, 751 23 Uppsala, Sweden

关键词: Complement;    Human C3;    Cyanogen bromide fragmentation;    Sequence;    Homology;    DITC;    p-phenylenediisothiocyanate;    HPLC;    high-pressure liquid chromatography;    C3α;    α-chain of human C3;    C3β;    β-chain of human C3;    CVF;    cobra venom factor;    CVFα;    α-chain of CVF;   

DOI  :  10.1016/0014-5793(84)80289-6

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The isolated β-chain of human complement factor C3 (C3β) was fragmented by cyanogen bromide. Nine fragments were defined by gel filtration and high-pressure liquid chromatogaphy, and characterized with respect to their M _r, amino acid composition and N-terminal amino acid sequence. Approx. 30% of the primary structure of C3β was determined. Alignment of the 3 N-terminal fragments allowed determination of 61 of the amino terminal residues of C3β. This region demonstrated 40% homology with the sequence in the N-terminal segment of the α-chain of the cobra venom factor.

【 授权许可】

Unknown