Transcriptional characteristics of in vitro assembled chromatin assayed by microinjection into Xenopus laevis oocytes

FEBS Letters

Steinbeißer, Herbert²  Trendelenburg, Michael F.²  Hofmann, Ansgar²  Oudet, Pierre¹ 

[1] Laboratoire de Génétique Moléculaire des Eukaryotes du CNRS, Faculté de Médecine, F-67085 Strasbourg Cédex, France;Institute of Experimental Pathology, German Cancer Research Center, Im Neuenheimer Feld 280, D-6900Heidelberg, FRG

关键词: Actin;    α-;    Oocyte injection;    Chromatin reorganization;   

DOI  :  10.1016/0014-5793(89)80660-X

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Plasmid DNA was in vitro assembled into chromatin using an S-150 extract of Xenopus laevis oocytes. By varying the assembly temperature and DNA concentration it is possible to generate fully or partially assembled molecules. The fate of the in vitro preassembled molecules injected into X. laevis oocyte nuclei and their transcriptional activity were studied. Completely reconstituted molecules underwent a rearrangement of their chromatin structure after injection and showed reduced transcriptional activity compared to protein-free DNA or partially reconstituted chromatin.

【 授权许可】

Unknown