Purification of squalene‐2,3‐epoxide cyclases from cell suspension cultures of Rabdosia japonica Hara

FEBS Letters

Sankawa, Ushio²  Ebizuka, Yutaka²  Seo, Shujiro¹  Abe, Ikuro² 

[1] Shionogi Research Laboratories, Shionogi & Co., Ltd, Fukushima-ku, Osaka 553, Japan;Faculty of Pharmaceutical Sciences, University of Tokyo, Hongo, Bunkyo-ku, Tokyo 113, Japan

关键词: Squalene-2;    3-epoxide;    Cycloartenol cyclase;    Amyrin cyclase;    β-;    Amyrin cyclase;    α-;    (Rabdosia japonica Hara);   

DOI  :  10.1016/0014-5793(89)80024-9

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Microsomes prepared from cell suspension cultures of Rabdosia japonica Hara showed activities for cyclizing squalene-2,3-epoxide into cycloartenol, β-amyrin and α-amyrin in the presence of Triton X-100. These activities were efficiently solubilized by treatment with Triton X-100 and separated by chromatography on hydroxy apatite, DEAE-cellulose, isoelectric focusing and gel filtration. The purified cycloartenol cyclase showed a single band on SDS-polyacrylamide gel electrophoresis with M_r = 54000, while β-amyrin cyclase gave a single band with M_r = 28000.

【 授权许可】

Unknown