Identification of the bovine brain Ins(1,4,5)P3 5‐phosphatase after SDS‐polyacrylamide gel electrophoresis

FEBS Letters

Lemos, Manuela¹  Dumont, Jacques E.¹  Erneux, Christophe¹ 

[1] Institut de Recherche Interdisciplinaire (IRIBHN), Faculté de Médecine, Université Libre de Bruxelles, Campus Erasme, Route de Lennik 808, 1070 Bruxelles, Belgium

关键词: Ca2+;    Inositol 1;    4;    5-trisphosphate 5-phosphatase;    (Bovine brain);    Ins(1;    4;    5)P3;    inositol 1;    4;    5-trisphosphate;    Ins(1;    3;    4;    5)P4;    inositol 1;    3;    4;    5-tetrakisphosphate;   

DOI  :  10.1016/0014-5793(89)80650-7

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Ins(1,4,5)P₃ 5-phosphatase catalyses the dephosphorylation of Ins(1,4,5)P₃ in the 5-position. In a high speed soluble fraction of bovine brain, there are two soluble 5-phosphatases: type I and type II. The purified Ins(1,4,5)P₃ 5-phosphatase type I exhibits a major silver-stained band of 43 kDa on denaturing (SDS) gels. It is possible to extract the 5-phosphatase activity form a duplicate lane after gel electrophoresis. The 43 kDa region contains the extractable Ins(1,4,5)P₃ 5-phosphatase activity.

【 授权许可】

Unknown