期刊论文详细信息
FEBS Letters | |
Identification of the bovine brain Ins(1,4,5)P3 5‐phosphatase after SDS‐polyacrylamide gel electrophoresis | |
Lemos, Manuela1  Dumont, Jacques E.1  Erneux, Christophe1  | |
[1] Institut de Recherche Interdisciplinaire (IRIBHN), Faculté de Médecine, Université Libre de Bruxelles, Campus Erasme, Route de Lennik 808, 1070 Bruxelles, Belgium | |
关键词: Ca2+; Inositol 1; 4; 5-trisphosphate 5-phosphatase; (Bovine brain); Ins(1; 4; 5)P3; inositol 1; 4; 5-trisphosphate; Ins(1; 3; 4; 5)P4; inositol 1; 3; 4; 5-tetrakisphosphate; | |
DOI : 10.1016/0014-5793(89)80650-7 | |
学科分类:生物化学/生物物理 | |
来源: John Wiley & Sons Ltd. | |
【 摘 要 】
Ins(1,4,5)P3 5-phosphatase catalyses the dephosphorylation of Ins(1,4,5)P3 in the 5-position. In a high speed soluble fraction of bovine brain, there are two soluble 5-phosphatases: type I and type II. The purified Ins(1,4,5)P3 5-phosphatase type I exhibits a major silver-stained band of 43 kDa on denaturing (SDS) gels. It is possible to extract the 5-phosphatase activity form a duplicate lane after gel electrophoresis. The 43 kDa region contains the extractable Ins(1,4,5)P3 5-phosphatase activity.
【 授权许可】
Unknown
【 预 览 】
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RO201912020292091ZK.pdf | 188KB | download |