Muramyl peptides bind specifically to rat brain membranes

FEBS Letters

Tsetlin, V.I.¹  Kaydalov, A.A.¹  Utkin, Yu.N.¹  Andronova, T.M.¹  Ivanov, V.T.¹ 

[1] Shemyakin Institute of Bioorganic Chemistry, USSR Academy of Sciences, Moscow V-437, USSR

关键词: Muramyl peptide;    Specific binding;    (Rat brain);    Azs;    4-azidosalicyl;    BSA;    bovine serum albumin;    GMDP;    N-acetylglucosaminylmuramyl dipeptide;    GMDP-Lys;    GMDP-Lys(Azs) and GMDP-Lys(Hp);    GMDP analogs with free or acylated lysine side chain;    Hp;    3-(4-hydroxyphenyl)propionyl;    MDP;    muramyl dipeptide;    Taps;    N-tris(hydroxymethyl)-3-aminopropanesulfonic acid;   

DOI  :  10.1016/0014-5793(89)80435-1

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The capability of immunoactive muramyl peptides to bind specifically to rat brain membranes has been discovered. The reaction of an N-acetylglucosaminylmuramyl dipeptide analog having an additional C-terminal lysine residue (GMDP-Lys) with Bolton-Hunter reagent or N-hydroxysuccinimidyl-4-azidosalicylate afforded two acylated derivatives, GMDP-Lys-(Hp) and GMDP-Lys(Azs). Their iodination resulted in radioactive derivatives (spec. act. ∼2000 Ci/mmol) whose binding to rat brain membranes is characterized by K _d∼3 nM and B _max∼10 fmol/mg membrane protein. Binding could be inhibited by muramyl dipeptide (MDP), GMDP-Lys, and GMDP, while fragments of the latter, N-acetylglucosamine, dipeptide and disaccharide, were ineffective.

【 授权许可】

Unknown