Endogenous tumour necrosis factor‐alpha sensitise melanoma cells to glucosaminylmuramyl dipeptide

FEBS Letters

Petrova, Elena E¹  Andronova, Tatyana M¹  Komaleva, Ravilya L¹  Valyakina, Tatyana I¹  Malakhov, Alexander A¹  Nesmeyanov, Vladimir A¹  Shamborant, Ol'ga G¹ 

[1] Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, ul. Miklukho-Maklaya 16/10, Moscow 117871, Russia

关键词: Tumour necrosis factor;    Muramyl peptide;    Melanoma-associated antigen;    GMDP;    N-acetylglucosaminyl-β1–4-N-acetylmuramyl-alanyl-d-isoglutamine;    MDP;    N-acetylmuramyl-alanyl-d-isoglutamine;    TNF-α;    tumour necrosis factor-alpha;    TGF-β1β2β3;    transforming growth factor-beta1;    2;    3;    MTT;    3-(4;    5-dimethylthiazol-2-yl)-2;    5-diphenyltetrazolium bromide;    mAb;    monoclonal antibody;    FCS;    foetal calf serum;    PBS;    phosphate-buffered saline;   

DOI  :  10.1016/S0014-5793(98)00376-7

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Flow cytometry was used to demonstrate that cultured human melanoma BRO cells expressed membrane-bound tumour necrosis factor-alpha (TNF-α) and were able to release TNF-α upon treatment with glucosaminylmuramyl dipeptide (GMDP). The released TNF-α was shown to prime melanoma cells, previously unable to respond to GMDP by increasing expression of melanoma-associated antigens, making them sensitive to GMDP treatment.

【 授权许可】

Unknown