Identity of GA1, GM1a and GD1b synthase in Golgi vesicles from rat liver

FEBS Letters

Sandhoff, Konrad¹  Iber, Heinrich¹  Schwarzmann, Günter¹  Kaufmann, Reinhard¹  Pohlentz, Gottfried¹ 

[1] Institut für Organische Chemie and Biochemie der Universität Bonn, Gerhard-Domagk-Straße 1, D-5300 Bonn 1, FRG

关键词: Glycosphingolipid;    Ganglioside;    Ganglioside synthesis;    Galactosyltransferase;    Cer;    ceramide (N-acylsphingosine);    Gal;    galactose;    GalNAc;    N-acetylgalactosamine;    Glc;    glucose;    NeuAc;    N-acetylneuraminic acid;    UDP-Gal;    uridine 5′-diphosphogalactose;    UDP-GalNAc;    uridine 5′-diphospho-N-acetylgalactosamine;    CDP-choline;    cytidine 5′-diphosphocholine;    CMP-NeuAc;    cytidine 5′-monophospho-N-acetylneuraminic acid;    GlcCer;    glucosylceramide;    Glc1 → 1Cer;    LacCer;    Galβ1 → 4Glcβ1 → 1Cer. In the nomenclature of Svennerholm [19] for gangliosides: G;    ganglioside;    M;    monosialo;    D;    disialo;    T;    trisialo;    and arabic numerals indicate the sequence of migration in thin-layer chromatograms;   

DOI  :  10.1016/0014-5793(89)80423-5

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Synthesis of ganglioside GD_D1b from ganglioside G_D2 was demonstrated using Golgi membranes isolated from rat liver. Competition experiments using gangliosides G_A2, G_M2 and G_D2 as substrates, and as mutual inhibitors for ganglioside galactosyltransferase activity in preparations of Golgi vesicles derived from rat liver, suggested that galactosyl transfer to these three compounds, leading to gangliosides G_A1, G_M1a and G_D1b respectively, is catalyzed by one enzyme. These results strengthen the hypothesis that the main site for the regulation of ganglioside biosynthesis occurs within the reaction sequence LacCer→G_A3→G_D3→G_T3.

【 授权许可】

Unknown