Determination of the functionally important heme peripheral vinyl group orientation in paramagnetic hemoprotein by 2D NMR

FEBS Letters

Chûjô, Riichirô¹  Suzuki, Tomohiko²  Inoue, Yoshio¹  Yamamoto, Yasuhiko¹  Ōsawa, Akemi¹ 

[1] Department of Biomolecular Engineering, Tokyo Institute of Technology, O-Okayama, Meguro-ku, Tokyo 152, Japan;Department of Biology, Faculty of Science, Kochi University, Kochi 780, Japan

关键词: NMR;    2D;    Heme;    Myoglobin;    Resonance assignment;    Vinyl group orientation;   

DOI  :  10.1016/0014-5793(89)81349-3

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

2D NMR spectroscopies have been successfully used to characterize the heme peripheral vinyl groups in paramagnetic hemoprotein in spite of the difficulties from the rapid paramagnetic relaxation and the low digital resolution of the 2D NMR map. The scalar coupling network system among the vinyl protons is clearly identified in the COSY spectra from its characteristic cross-peak pattern and the dipolar coupling connectivities of the vinyl proton resonances with other heme side-chain proton resonances not only provide the specific assignment of vinyl β-proton resonances but also allow the determination of the vinyl group orientation with respect to the heme plane.

【 授权许可】

Unknown