Proton NMR study of the influence of heme vinyl groups on the formation of the isomeric forms of sulfmyoglobin

FEBS Letters

La Mar, Gerd N.¹  Parish, Daniel W.¹  Chatfield, Mariann J.¹  Balch, Alan.L.¹  LePage, Teresa J.¹  Smith, Kevin M.¹ 

[1] Department of Chemistry, University of California, Davis, CA 95616, USA

关键词: Sulfmyoglobin;    1H-NMR;    Heme;    Vinyl group;    Myoglobin;    SulfMb;    sulfmyoglobin;    SAMb;    SBMb;    ScMb;    green forms of sulfmyoglobin;    SDMb;    SEMb;    red forms of sulfmyoglobin;   

DOI  :  10.1016/0014-5793(86)81009-2

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The formation of sulfmyoglobin has been investigated for myoglobin reconstituted with hemins having vinyls replaced by hydrogens to determine the participation of the vinyl groups in the reaction processes. Green complexes are produced in all cases, proving that vinyls are not obligatory for the formation of sulfproteins. In the presence of the 4-vinyl group, the ¹H NMR spectra of the met-cyano derivatives indicate the formation of three green species; however, the most stable of these products is not formed in the absence of this group, confirming reaction of the 4-vinyl in this species. Two new red extractable sulfmyoglobin derivatives are formed in the absence of the 4-vinyl group.

【 授权许可】

Unknown