Assignment of hyperfine shifted haem methyl carbon resonances in paramagnetic low‐spin met‐cyano complex of sperm whale myoglobin

FEBS Letters

Yamamoto, Yasuhiko¹ 

[1] Department of Polymer Chemistry, Tokyo Institute of Technology, O-okayama, Meguro-ku, Tokyo 152, Japan

关键词: 13C-NMR;    Myoglobin;    Hyperfine shift;    Heme;    (Sperm whale);    NMR;    nuclear magnetic resonance;    DSS;    2;    2-dimethyl-2-silapentane-5-sulphonate;    1H-13C COSY;    1H-13C heteronuclear chemical shift correlated spectroscopy;    Mb;    myoglobin;    metMbCN;    met-cyano myoglobin;   

DOI  :  10.1016/0014-5793(87)80202-8

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The hyperfine shifted resonances arising from all four individual haem carbons of the paramagnetic low-spin met-cyano complex of sperm whale myoglobin have been clearly identified and assigned for the first time with the aid of ¹H-¹³C heteronuclear chemical shift correlated spectroscopy. Alteration of the in-plane symmetry of the electronic structure of haem induced by the ligation of proximal histidyl imidazole spreads the haem carbon resonances to 32 ppm at 22°C, indicating the sensitivity of those resonances to the haem electronic/molecular structure. Those resonances are potentially powerful probes in characterizing the nature of haem electronic structure.

【 授权许可】

Unknown