Stimulation of polyphosphoinositide hydrolysis in Swiss 3T3 cells by recombinant fibroblast growth factors

FEBS Letters

Brigstock, David R.¹  Brown, Kenneth D.¹  Blakeley, Diana M.¹ 

[1] Department of Biochemistry, AFRC Institute of Animal Physiology and Genetics Research, Babraham, Cambridge CB2 4AT, England

关键词: Phosphatidylinositol turnover;    Ca2+ mobilization;    Fibroblast growth factor;    Bombesin;    FGF;    fibroblast growth factor;    IPs;    inositol phosphates;    PBS;    phosphate-buffered saline;    TPA;    12-O-tetradecanoyl-phorbol-13-acetate;   

DOI  :  10.1016/0014-5793(89)81340-7

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Mitogenic concentrations of recombinant acidic or basic fibroblast growth factor (FGF) stimulated the accumulation of [³H]inositol phosphates ([³H]IPs) in Swiss 3T3 cells pre-labelled for 48 h with [³H]inositol. Maximal effects were obtained at 0.3 ng/ml and 3 ng/ml for basic and acidic FGF, respectively. Higher doses of either factor led to a diminished stimulation. FGF also stimulated ⁴⁵Ca²⁺ release from cells pre-labelled with the isotope. However, FGF-stimulated production of [³H]IPs and release of ⁴⁵Ca²⁺ exhibited marked differences when compared with the responses to the peptide mitogen bombesin; the FGF responses were markedly slower and were not inhibited by phorbol esters.

【 授权许可】

Unknown