Hormone‐sensitive lipase is responsible for the neutral cholesterol ester hydrolase activity in macrophages

FEBS Letters

Small, Catherine A.²  Yeaman, Stephen J.²  Goodacre, John A.¹ 

[1] Department of Rheumatology, University of Newcastle upon Tyne, Newcastle upon Tyne NE2 4HH, England;Department of Biochemistry and Genetics, University of Newcastle upon Tyne, Newcastle upon Tyne NE2 4HH, England

关键词: Hormone-sensitive lipase;    Cholesterol ester hydrolase;    Macrophage;    Atherosclerosis;    ACAT;    acyl CoA:cholesterol acyltransferase;    HDL;    high-density lipoprotein;    HSL;    hormone-sensitive lipase;    LDL;    low-density lipoprotein;   

DOI  :  10.1016/0014-5793(89)81335-3

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Anti-hormone-sensitive lipase (HSL) immunoglobulin selectively immunoprecipitates a single 84 kDa ³²P-phosphoprotein from macrophage homogenates previously phosphorylated by cyclic AMP-dependent protein kinase in the presence of [γ-³²P]ATP-Mg. This immunoglobulin also completely removes the neutral cholesterol ester hydrolase activity from macrophage homogenates. These data demonstrate that HSL is responsible for the neutral cholesterol ester hydrolase activity in macrophages and hence plays a key role in cholesterol metabolism in these cells.

【 授权许可】

Unknown