Carboxypeptidase‐B‐like processing of the C‐terminus of glucagon‐like peptide‐2 in pig and human small intestine

FEBS Letters

Buhl, T.¹  Ørskov, C.¹  Kofod, H.²  Rabenhøj, L.¹  Holst, J.J.¹ 

[1] Institute of Medical Physiology C, Panum Institute, Copenhagen Denmark;Hagedorn Research Laboratory, Gentofte, Denmark

关键词: Proglucagon;    Posttranslational processing;    Glucagon;    Enteroglucagon;    Gastrointestinal hormone;   

DOI  :  10.1016/0014-5793(89)81332-8

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

We developed specific, C-terminal radioimmunoassays for three proglucagon (PG) fragments: PG 151–158, PG 151–160 and PG 126–159 (glucagon-like peptide-2 (GLP-2)) in order to determine the exact C-terminal sequence of the newly isolated GLP-2 in man and pig. The antigens and the antisera showed no mutual cross-reactivity. By gel filtration of extracts of pig and human small intestine, the immunoreactivity eluting at the position of GLP-2 was identified by the radioimmunoassays for glucagon-like peptide-2 (PG 126–159) and for PG 151–158, whereas the assay for PG 151–160 was completely negative. We conclude that the C-terminal amino acid residue of pig and human ileal GLP-2 is PG 158. Thus the basic residues, PG 159 and 160 are removed during its processing in the small intestine.

【 授权许可】

Unknown