Truncated glucagon‐like peptide‐1 (proglucagon 78–107 amide), an intestinal insulin‐releasing peptide, has specific receptors on rat insulinoma cells (RIN 5AH)

FEBS Letters

Ørskov, Cathrine¹  Nielsen, Jens Høiriis¹ 

[1] Hagedorn Research Laboratory, Gentofte, Denmark

关键词: Proglucagon;    Enteroglucagon;    Incretin;    Insulinotropin;   

DOI  :  10.1016/0014-5793(88)80821-4

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

We studied binding of ¹²⁵I-labelled truncated-glucagon-like peptide-1 (proglucagon 78–107 amide) to a cloned rat insulin-producing cell line, RIN 5AH, in monolayer culture. Interaction of the peptide with pancreatic insulinoma cells was saturable and time dependent. Half-maximal binding was obtained when the cells were incubated in the presence of 3.3 × 10⁻⁹ mol/l unlabelled truncated-glucagon-like peptide-1 (proglucagon 78–107 amide). Neither glucagon, full-length glucagon-like peptide-1 (proglucagon 72–107 amide) nor gastric inhibitory peptide competed for binding in concentrations up to 10⁻⁶ mol/l.

【 授权许可】

Unknown