Nonmuscle actin ADP‐ribosylated by botulinum C2 toxin caps actin filaments

FEBS Letters

Weigt, Christiane¹  Just, Ingo²  Aktories, Klaus²  Wegner, Albrecht¹ 

[1] Institute of Physiological Chemistry, Ruhr-University Bochum, D-4630 Bochum FRG;Rudolf-Buchheim-Institute of Pharmacology, D-6300 Giessen, FRG

关键词: Botulinum C2 toxin;    ADP-ribosylation;    Actin;    Capping protein;   

DOI  :  10.1016/0014-5793(89)80279-0

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The effect of nonmuscle actin ADP-ribosylated by botulinum C2 toxin on the polymerization of nonmuscle actin was investigated in order to clarify whether nonmuscle actin is converted into a capping protein by ADP-ribosylation. ADP-ribosylated actin was found to decrease the rate of polymerization of actin filaments which are free at both ends. ADP-ribosylated actin turned out to have no effect on the rate or extent of polymerization at the pointed ends of actin filaments the barbed ends of which were capped by gelsolin. The monomer concentration reached at the final stage of polymerization was similar to the critical concentration of the pointed ends of actin filaments. The results suggest that nonmuscle actin ADP-ribosylated by botulinum C2 toxin acts as a capping protein which binds to the barbed ends to inhibit polymerization.

【 授权许可】

Unknown