Clostridium perfringens iota toxin ADP‐ribosylates skeletal muscle actin in Arg‐177

FEBS Letters

Schering, Beate¹  Vandekerckhove, Joël²  Bärmann, Michael¹  Aktories, Klaus¹ 

[1] Institut für Pharmakologie und Toxikologie D-6300 Giessen, FRG;Laboratorium voor Genetica, Rijksuniversiteit Gent, B-9000 Gent, Belgium

关键词: ADP-ribosylation;    Actin;    Iota toxin;    C2 toxin;    (C. perfringens;    C. botulinum);   

DOI  :  10.1016/0014-5793(87)81129-8

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Clostridium perfringens iota toxin ADP-ribosylates actin. Substrates of C. perfringens toxin are both non-muscle β/γ-actin and skeletal muscle actin. This finding suggests that C. perfringens iota ADP-ribosylates the same amino acid in skeletal muscle and non-muscle actin as does C. botulinum C2 toxin in non-muscle actin. Protein chemical analysis involving thermolysin cleavage on [³²P]ADP-ribosylated actin or tryptic digestion followed by a secondary thermolysin cleavage of the radiolabelled fragments showed one major site of ADP-ribosylation. From its amino acid composition and sequence, the radiolabelled peptide was identified as peptide 175–177, locating the acceptor ADP-ribosyl amino acid as Arg-177.

【 授权许可】

Unknown