‘Cap 90’, a 90‐kDa Ca2+‐dependent F‐actin‐capping protein from vertebrate brain

FEBS Letters

Isenberg, G.¹  Ohnheiser, R.¹  Maruta, H.¹ 

[1] Max Planck Institute for Psychiatry, Department of Neurochemistry, Am Klopferspitz 18a, 8033 Martinsried, FRG

关键词: Actin;    Capping protein;    Ca2+;    Bovine brain;    Cytoskeleton;    Triton model;    DNase-I;    deoxyribonuclease I;    EGTA;    ethyleneglycol bis (β-aminoethylether)-N;    N;    N′;    N′-tetraacetic acid;    PMSF;    phenylmethylsulfonylfluoride;    SDS—PAGE;    sodium dodecyl sulfate—polyacrylamide gel electrophoresis;    S-1;    myosin subfragment-1;   

DOI  :  10.1016/0014-5793(83)80824-2

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

A Ca²⁺-dependent actin filament-capping protein of 90 kDa was purified from bovine brain using a new and rapid isolation procedure. This basically includes affinity purification on DNase-I agarose. The protein caps the fast-growing end of actin filaments but has no fragmenting or severing activity. Using Triton X-100-extracted cytoskeletons, capping and severing activities of actin-binding proteins become clearly distinguishable from each other.

【 授权许可】

Unknown