Generation of O− 2 and tyrosine cation‐mediated chemiluminescence during the fertilization of sea urchin eggs

FEBS Letters

Totsune-Nakano, Hiroko³  Suzuki, Nobutaka³  Takahashi, Atsushi³  Inaba, Humio²  Ohma, Chikako²  Nakano, Minoru¹  Mashiko, Shinro³ 

[1] College of Medical Care and Technology, Gunma University, Maebashi, Gunma Japan;Research Institute of Electrical Communication, Tohoku University, Sendai, Miyagi, Japan;Inaba Bio-Photon Project, Research Development Corporation of Japan, Kojinkai Central Hospital, 2-1-6 Tsutsujigaoka, Sendai, Miyagi, Japan

关键词: Fertilization;    Superoxide;    Chemiluminescence;    (Sea urchin egg);   

DOI  :  10.1016/0014-5793(89)80265-0

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Sea urchin eggs emit light in the visible region during their fertilization. Judging from the chemiluminescence spectra, one of the excited species generated is considered to have originated from a tyrosine cation radical-mediated reaction. Chemiluminescence probes such as luminol or a cypridina luciferin analog (2-methyl-6-[p-methoxyphenyl]-3,7-dihydroimidazo-[1,2-a]pyrazin-3-one) are useful for detecting the ovoperoxidase⁺H₂O₂ reaction associated with membrane hardening and O⁻ ₂ generation, respectively, during the fertilization of sea urchin eggs.

【 授权许可】

Unknown