Effect of rutin and its copper complex on superoxide formation and lipid peroxidation in rat liver microsomes

FEBS Letters

Afanas'ev, Igor B²  Korkina, Ludmila G¹  Ostrachovich, Elena A² 

[1] Russian Medical University, Ostrovityanova str. 1, Moscow 117513, Russia;Vitamin Research Institute, Nauchny pr. 14A, GSP-7, Mowcow 117820, Russia

关键词: Superoxide;    Chemiluminescence;    Microsomal lipid peroxidation;    CL;    chemiluminescence;    CuZnSOD;    copper;    zinc superoxide dismutase;    TBA;    thiobarbituric acid;    TBAR products;    thiobarbituric acid reaction products;   

DOI  :  10.1016/S0014-5793(98)00244-0

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Two free radical scavengers, bioflavonoid rutin and the copper-rutin complex Cu(Rut)Cl₂, inhibited lucigenin-amplified chemiluminescence and lipid peroxidation in rat liver microsomes, Cu(Rut)Cl₂ being a 5–9 times more efficient inhibitor than rutin. The enhanced inhibitory activity of Cu(Rut)Cl₂ was due to the presence of the additional superoxide-dismutating center (Cu), as follows from the comparison of its effects on microsomal chemiluminescence and cytochrome c reduction by xanthine oxidase. Similar effects of both inhibitors on superoxide production and lipid peroxidation as well as the elevated activity of Cu(Rut)Cl₂ indicate an important role of superoxide ion in the initiation of microsomal lipid peroxidation.

【 授权许可】

Unknown