Age related impairment in phosphatidylinositol breakdown of polymorphonuclear granulocytes

FEBS Letters

Leövey, A.¹  Fóris, G.¹  Fülöp, T.¹  Varga, Z.¹  Csongor, J.¹ 

[1] Institute Department of Medicine, University Medical School of Debrecen, 4012 Debrecen, POB 19, Hungary

关键词: Aging;    Inositol phosphate;    GTPγS;    (Granulocyte);    IP1;    inositol 4-monophosphate;    IP2;    inositol 1;    4-bisphosphate;    IP3;    inositol 1;    4;    5-trisphosphate;    GTPγS;    guanosine-5′-O-(3-thiotriphosphate);    PIP2;    phosphatidylinositol 4;    5-bisphosphate;    DAG;    diacylglycerol;    PKC;    protein kinase C;    PLC;    phospholipase C;    PLA2;    phospholipase A2;    FMLP;    N-formyl-methionyl-leucyl-phenylalanine;    PMNLs;    polymorphonuclear granulocytes;    PT;    pertussis toxin;   

DOI  :  10.1016/0014-5793(89)80231-5

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

It is well known that with aging the immune response decreases. Most of the effector functions occur through specific receptors. Thus, we investigated the effects of various stimulants, acting through receptors or directly through the GTP-binding G_i protein, on phosphatidylinositol breakdown in PMNLs of young and elderly subjects and try to modulate it. A marked decrease in inositol phosphate (IP₁, IP₂, IP₃) formation in PMNLs of elderly was found under FMLP stimulation when compared to that of young subjects. Neither GTPγS, nor AIF⁻ ₄ could induce an increase of IP₃ in PMNLs of elderly comparable to that of young subjects. The results suggest that at least an alteration exists at the GTP-binding G_i protein level, as well as in the mechanism of linkage of the receptor to the G protein.

【 授权许可】

Unknown