Okadaic acid, a specific protein phosphatase inhibitor, induces maturation and MPF formation in Xenopus laevis oocytes

FEBS Letters

Goris, Jozef²  Merlevede, Wilfried²  Hermann, Jacques¹  Hendrix, Peter²  Ozon, René¹ 

[1] Laboratoire de Physiologie de la Reproduction, Université Pierre et Marie Curie, 75252 Paris, France;Afdeling Biochemie, Faculteit Geneeskunde, Katholieke Universiteit Leuven, B-3000 Leuven, Belgium

关键词: Okadaic acid;    Oocyte maturation;    Protein phosphatase;    OA;    okadaic acid;    MPF;    maturation-promoting factor;    GVBD;    germinal vesicle breakdown;    PCS;    polycation-stimulated;    AMD;    ATP;    Mg-dependent;    cdc;    cell division cycle;    pNPP: p-nitrophenyl phosphate;   

DOI  :  10.1016/0014-5793(89)80198-X

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Micro-injection of, or incubation with okadaic acid (OA), a specific phosphatase inhibitor, can induce formation of maturation-promoting factor (MPF) and germinal vesicle breakdown (GVBD) in Xenopus laevis oocytes. Comparison of the dose-response curves of OA on maturation, isolated enzymes and phosphatase activities in crude oocyte preparations suggests that inhibition of both polycation-stimulated (PCS) and ATP,Mg-dependent (AMD) phosphatases is sufficient but requires that a critical phosphorylation level is attained of one or several of their substrates, resulting in the formation of active MPF and meiotic maturation.

【 授权许可】

Unknown