Cytochrome aa 3 from the thermoacidophilic archaebacterium Sulfolobus acidocaldarius

FEBS Letters

Anemüller, S.¹  Schäfer, G.¹ 

[1] Institute of Biochemistry, Medical University of Lübeck, Ratzeburger Allee 160, D-2400 Lübeck, FRG

关键词: Archaebacteria;    Electron transport;    Hemoprotein;    Copper;    Midpoint potential;    Caldariella quinone;   

DOI  :  10.1016/0014-5793(89)80581-2

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Cytochrome aa ₃ serves as a terminal oxidase in the thermoacidophilic archaebacterium Sulfolobus acidocaldarius. A procedure for its isolation is described. The purified preparation consists of only one major polypeptide of 38 kDa apparent molecular mass. The enzyme contains two heme α molecules with midpoint potentials of + 220 and + 370 mV, respectively. The copper content is at least 2 Cu/aa ₃. It has only negligible capacity to oxidize cytochrome c, but rather serves as an oxidase for reduced caldariella quinone as present in the membrane of Sulfolobus.

【 授权许可】

Unknown