Formation and release of nitric oxide from human neutrophils and HL‐60 cells induced by a chemotactic peptide, platelet activating factor and leukotriene B4

FEBS Letters

Böhme, Eycke¹  Seifert, Roland¹  Schmidt, Harald H.H.W.¹ 

[1] Institut für Pharmakologie, Freie Universität Berlin, Thielallee 69-73, D-1000 Berlin 33, Germany

关键词: Nitric oxide;    Neutrophil-derived relaxing factor;    NADPH oxidase;    Arginine;    (Neutrophil;    HL-60 cell);    CAN;    L-canavanine sulfate;    dbcAMP;    dibutyryl cyclic AMP;    EDRF;    endothelium-derived relaxing factors;    FMLP;    N-formyl-L-methionyl-L-leucyl-L-phenylalanine;    LTB4;    leukotriene B4;    NDRF;    neutrophil-derived relaxing factors;    1;    25(OH)2D3;    1;    25-dihydroxy-vitamin D3;    PAF;    platelet activating factor;    SOD;    superoxide dismutase;   

DOI  :  10.1016/0014-5793(89)80562-9

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Vascular endothelial cells and neutrophils synthesize and release potent vasodilatatory factors, i.e. endothelium-derived relaxing factors (EDRF) and neutrophil-derived relaxing factors (NDRF). One EDRF has been identified as nitric oxide (NO) derived from arginine. We studied the synthesis and release of NO from human neutrophils stimulated with the chemotactic peptide N-formyl-L-methionyl-L-leucyl-L-phenylalanine, platelet activating factor or leukotriene B₄. The formation and release of NO was enhanced several-fold in the presence of superoxide dismutase, probably by inhibiting superoxide-induced breakdown of NO. The formation and release of NO but not the formation of superoxide anions was decreased in neutrophils pretreated with L-canavanine, an inhibitor of arginine-utilizing enzymes. Our data suggest that at least one NDRF is identical with NO or another labile NO containing compound derived from arginine.

【 授权许可】

Unknown