Squalene epoxide cyclase and microemulsion

FEBS Letters

Duriatti, Albert¹  Lee, Kang Min²  Biellmann, Jean-François²  Schuber, Francis¹ 

[1] UA-1182, Laboratoire de Chimie Enzymatique, Institut de Botanique, Université Louis Pasteur, 28 Rue Goethe, 67000 Strasbourg Cedex, France;UA-31, Laboratoire de Chimie Organique Biologique, Institut de Chimie, Université Louis Pasteur, 1 Rue Blaise Pascal, 67008 Strasbourg Cedex, France

关键词: Squalene epoxide cyclase;    Microemulsion;    Micelle;    Detogert;    (Rat liver);   

DOI  :  10.1016/0014-5793(89)80560-5

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Squalene epoxide cyclase was extracted from microsomal preparations of rat liver using anionic, cationic and non-ionic microemulsions. The anionic microemulsion was the best with respect to protein solubilisation, extracted cyclase activity and stability of this activity over time. The activity assay showed cyclase activity to be higher in anionic microemulsion than in buffer in the presence of surfactant. Calcium chloride in the anionic microemulsion had a stabilising effect and less total protein seemed to be extracted.

【 授权许可】

Unknown