A cytoplasmic peptide of the neurotrophin receptor p75NTR: induction of apoptosis and NMR determined helical conformation

FEBS Letters

Assa-Munt, Nuria³  Rabizadeh, S.³  Krishnan, V.V.³  Bredesen, Dale³  Hileman, Matthew R.²  Chapman, Barbara S.¹  Plesniak, Leigh A.² 

[1] Biological Sciences, California State University, San Marcos, San Marcos, CA 92096, USA;Department of Chemistry, University of San Diego, 5998 Acala Park, San Diego, CA 92110, USA;Burnham Institute, La Jolla, CA 92037, USA

关键词: Micelle;    Spin label;    Chemical shift index;    CSI;    chemical shift indexing;    dNN;    NOE cross peak between adjacent amide protons;    DODPC;    dodecylphosphocholine-d38;    DQF-COSY;    double quantum filtered correlated spectroscopy;    FBS;    fetal bovine serum;    L373K;    lysine variant of NTR368;    NOESY;    nuclear Overhauser effect spectroscopy;    NGFR;    nerve growth factor receptor;    SH-SY;    SH-SY-5Y human neuroblastoma cells;    NTR;    p75 neurotrophin receptor;    NTR367;    14 amino acid peptide containing NTR residues 367–380;    NTR368-L373K;    14 amino acid peptide containing residues 367–380 of human NTR sequence with lysine substitution at residue 373;    NTR368;    14 amino acid fragment of NTR containing residues 368–381;    rmsd;    root mean square deviation;    SDS;    sodium dodecyl sulfate;    SFM;    serum free medium;    tat-NTR368;    peptide fusion of tat protein with NTR368;    tat-NTR368-L373K;    fusion peptide containing tat protein sequence and the lysine variant of NTR368;    TNFR;    tumor necrosis factor receptor;    TOCSY;    total correlation spectroscopy;   

DOI  :  10.1016/S0014-5793(97)01113-7

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The neurotrophin receptor (NTR) and tumor necrosis factor receptor family of receptors regulate apoptotic cell death during development and in adult tissues [Beutler and van Huffel, Science 264 (1994) 667–668]. We have examined a fragment of p75NTR from the carboxyl terminus of the receptor and a variant form of this peptide via NMR techniques and in vitro assays for apoptotic activity. The wild type peptide induces apoptosis and adopts a helical conformation oriented parallel to the surface of lipid micelles, whereas the variant form adopts a non-helical conformation in the presence of lipid and shows no activity. These experiments suggest a link between structure and function of the two peptides.

【 授权许可】

Unknown