A general method for the preparation of mixed micelles of hydrophobic peptides and sodium dodecyl sulphate

FEBS Letters

Chupin, Vladimir²  Lindblom, Göran³  Killian, J.Antoinette²  Trouard, Theodore P.³  Greathouse, Denise V.¹ 

[1]Department of Chemistry and Biochemistry, University of Arkansas, Fayetteville, AR 72701, USA

[2]Department of Biochemistry of Membranes, University of Utrecht, Padualaan 8, 3584CH Utrecht, The Netherlands

[3]Department of Physical Chemistry, University of Umeå, Umeå, Sweden

关键词: Micelle;    Two-dimensional NMR;    Circular dichroism;    Peptide structure;    Signal peptide;    Gramicidin;    BOC;    N-α-butyloxycarbonyl;    CD;    circular dichroism;    2D-NMR;    two-dimensional nuclear magnetic resonance;    FMOC;    fluorenylmethyloxycarbonyl;    HFP;    hexafluoroisopropanol;    HOBT;    1-hydroxybenzotriazole;    OD;    optical density;    SDS;    sodium dodecyl sulphate;    TFA;    trifluoroacetic acid;    TFE;    trifluoroethanol;   

DOI  :  10.1016/0014-5793(94)00594-X

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

A new method is reported for the incorporation of hydrophobic peptides into sodium dodecyl sulphate (SDS) micelles. First, a homogeneous solution of peptide and detergent is obtained by adding the peptide in trifluoroethanol to an equal volume of an aqueous solution of SDS. Upon subsequent addition of excess water, mixed peptide-SDS micelles are formed. Next, all solvent is removed by lyophilization and an appropriate amount of water is added to the dry powder. For various hydrophobic peptides this was shown to yield clear and stable solutions that are highly concentrated and suitable for characterization by spectroscopic techniques.

【 授权许可】

Unknown