NMR and circular dichroism studies of the lantibiotic nisin in non‐aqueous environments

FEBS Letters

Rollema, Harry S.¹  van de Ven, Frank J.M.²  van den Hooven, Henno W.²  Fogolari, Federico²  Hilbers, Cornelis W.²  Konings, Ruud N.H.² 

[1] Department of Biophysical Chemistry, Netherlands Institute for Dairy Research (NIZO), Kernhemseweg 2, 6718 ZB Ede, The Netherlands;Nijmegen Son Research Center (NSR Center), Laboratory of Biophysical Chemistry, University of Nijmegen, Toernooiveld, 6525 ED Nijmegen, The Netherlands

关键词: Bacteriocin;    Lantibiotic;    Nisin;    NMR;    Two-dimensional;    Circular dichroism;    Micelle;    Dha;    dehydroalanine;    Dhb;    dehydrobutyrine (3-methyldehydroalanine);    TFE;    trifluoroethanol;    SDS;    sodium dodecyl sulfate;    DPC;    dodecylphosphocholine;    CMC;    critical micelle concentration;    RP-HPLC;    reversed phase-high performance liquid chromatography;    CD;    circular dichroism;    2D NMR;    two-dimensional nuclear magnetic resonance;    NOE;    nuclear Overhauser enhancement;    NOESY;    nuclear Overhauser enhancement spectroscopy;    TOCSY;    total correlated spectroscopy;    P.COSY;    purged correlated spectroscopy;   

DOI  :  10.1016/0014-5793(93)80065-3

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The lantibiotic, nisin, which is known to interact with membranes of certain Gram-positive bacteria, was studied in three model systems which mimic a membrane-like environment, i.e. a mixture of trifluoroethanol and water, or micelles of sodium dodecyl sulfate or dodecylphosphocholine. The ¹H NMR spectra of nisin in the non-aqueous environments, at 40°C and pH 3.5, have been assigned completely. The CD and NMR results indicate that the conformation of nisin in the three non-aqueous environments differs from that in aqueous solution, and that the conformation in the two micellar systems is similar. The major conformational changes, relative to nisin in aqueous solution, occur in the N-terminus.

【 授权许可】

Unknown