In vivo activity of Nisin A and Nisin V against Listeria monocytogenesin mice

BMC Microbiology

Research Article

Des Field¹  Alicia Campion¹  Colin Hill²  Pat G Casey²  R Paul Ross³  Paul D Cotter³ 

[1] Department of Microbiology, University College Cork, Cork, Ireland;Department of Microbiology, University College Cork, Cork, Ireland;Alimentary Pharmabiotic Centre, University College Cork, Cork, Ireland;Teagasc, Moorepark Food Research Centre, Fermoy, Co, Cork, Ireland;Alimentary Pharmabiotic Centre, University College Cork, Cork, Ireland;

关键词: Antimicrobial;    Lantibiotic;    Bacteriocin;    Peptide engineering;    Mutagenesis;    Nisin;   

DOI  :  10.1186/1471-2180-13-23

 received in 2012-10-10, accepted in 2013-01-30,  发布年份 2013

来源: Springer

【 摘 要 】

BackgroundLantibiotics are post-translationally modified antimicrobial peptides, of which nisin A is the most extensively studied example. Bioengineering of nisin A has resulted in the generation of derivatives with increased in vitro potency against Gram-positive bacteria. Of these, nisin V (containing a Met21Val change) is noteworthy by virtue of exhibiting enhanced antimicrobial efficacy against a wide range of clinical and food-borne pathogens, including Listeria monocytogenes. However, this increased potency has not been tested in vivo.ResultsHere we address this issue by assessing the ability of nisin A and nisin V to control a bioluminescent strain of Listeria monocytogenes EGDe in a murine infection model.More specifically, Balb/c mice were infected via the intraperitoneal route at a dose of 1 × 105 cfu/animal and subsequently treated intraperitoneally with either nisin V, nisin A or a PBS control. Bioimaging of the mice was carried out on day 3 of the trial. Animals were then sacrificed and levels of infection were quantified in the liver and spleen.ConclusionThis analysis revealed that nisin V was more effective than Nisin A with respect to controlling infection and therefore merits further investigation with a view to potential chemotherapeutic applications.

【 授权许可】

Unknown   
© Campion et al.; licensee BioMed Central Ltd. 2013. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.