Pertussis toxin activates protein kinase C and a tyrosine protein kinase in the human T cell line Jurkat

FEBS Letters

Thom, Rebecca E.¹  Casnellie, John E.¹ 

[1] Department of Pharmacology & Cancer Center, University of Rochester, School of Medicine and Dentistry, 601 Elmwood Avenue, Rochester, NY 14642, USA

关键词: Tyrosine phosphorylation;    Pertussis toxin;    (T cell);    PHA;    phytohemagluttinin;    pp56lck;    T cell-specific tyrosine protein kinase of M r 56 000;    PMA;    phorbol myristic acetate;   

DOI  :  10.1016/0014-5793(89)81188-3

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Pertussis toxin activates T lymphocytes by a mechanism that is independent of its ADP-ribosylation activity. The toxin stimulates increases in diacylglycerol and intracellular calcium apparently by interacting with a cell surface receptor. Consistent with the production of these second messengers we have found that pertussis toxin activates protein kinase C in the Jurkat cell line. The toxin was also found to activate a tyrosine protein kinase in these cells in a manner similar to that observed with phytohemagluttinin. These results provide evidence that the mechanism of activation of T cells by pertussis toxin involves stimulating the activity of protein kinase C and a tyrosine protein kinase.

【 授权许可】

Unknown